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Algal Nutrient Limitation Bioassays in Sycamore Creek, Arizona, USA (2010-2020)

Publication date: 2020-08-28

Author(s):

  • Nancy Grimm, Arizona State University
  • John Sabo, Arizona State University
  • Sophia Bonjour, Arizona State University
  • Xiaoli Dong, University of California, Davis
  • Amalia Handler, USEPA National Health and Environmental Effects
  • Katherine Kemmitt, Wenck
  • Marina Lauck, Arizona State University
  • Monica Palta, Pace University
  • Lindsey Pollard, Arizona State University

Abstract:

The primary objective of this project is to understand how long-term climate variability and change influence the structure and function of desert streams via effects on hydrologic disturbance regimes. Climate and hydrology are intimately linked in arid landscapes; for this reason, desert streams are particularly well suited for both observing and understanding the consequences of climate variability and directional change. Researchers try to (1) determine how climate variability and change over multiple years influence stream biogeomorphic structure (i.e., prevalence and persistence of wetland and gravel-bed ecosystem states) via their influence on factors that control vegetation biomass, and (2) compare interannual variability in within-year successional patterns in ecosystem processes and community structure of primary producers and consumers of two contrasting reach types (wetland and gravel-bed stream reaches). This dataset addresses patterns of nutrient limitation by primary producers as indicated by *in situ* growth on artificial substrates.

Keywords:


Temporal Coverage:

2010-05-15 to 2020-05-21

Geographic Coverage:

Geographic Description: gravel reach of the Dos S (SS) sampling site of Sycamore Creek in central Arizona, USA
Bounding Coordinates:
Longitude:-111.507645 to -111.506849
Latitude:33.750482 to 33.749021

Geographic Coverage:

Geographic Description: wetland reach of the Dos S (SS) sampling site of Sycamore Creek in central Arizona, USA
Bounding Coordinates:
Longitude:-111.510883 to -111.50779
Latitude:33.748620 to 33.74835

Contact:

Information Manager, Central Arizona–Phoenix LTER, 
Arizona State University,Global Institute of Sustainability,Tempe
 caplter.data@asu.edu

Methods used in producing this dataset: Show


Data Files (1) :

Tabular: 590_nutrient_diffusing_substrata_fa70c9a52cdf9b19a09feb11ad21e93c.csv

Description: nutrient diffusion assays to assess nutrient limitation of primary producers in wetland and gravel sites of Sycamore Creek, AZ

Column Description Type Units
Date_retrieved Date cups were recovered from field incubations
date Format: YYYY-MM-DD
Site Location of field incubation
string
Enumeration:
  • SSG: DosS gravel reach
  • SSW: DosS wetland reach
Days_in_field Days of field incubation
float nominalDay
Treatment Agar treatment used: Control- agar only, NO3- agar and sodium nitrate, PO4- agar and potasium phosphate monobasic, NO3+PO4- agar, sodium nitrate, and potasium phosphate monobasic. A cation study was done in 2015 and included sodium phosphate monobasic
string
Enumeration:
  • Control: agar only
  • NaP: agar and sodium phosphate monobasic
  • NO3: agar and sodium nitrate
  • NO3+NaP: agar sodium nitrate and sodium phosphaphate monobasic
  • NO3+PO4: agar sodium nitrate and potasium phosphate monobasic
  • PO4: agar and potasium phosphate monobasic
Extract Either ethanol or methanol was used
string
Enumeration:
  • Ethanol: Ethanol
  • Methanol: Methanol
Extract_vol_ml Volume of solvent (methanol) used in exctraction
float milliliter
ABS_750 Background corrected absorbance measurement on spectrophotometer at wavelength 750 nm. Negative values were changed to zero
float nanometer
ABS_666 Background corrected absorbance measurement on spectrophotometer at wavelength 666 nm, 665 nm was used for ethanol extractions
float nanometer
ABS_480 Background corrected absorbance measurement on spectrophotometer at wavelength 480 nm
float nanometer
ABS_750a Background corrected absorbance measurement on spectrophotometer at wavelength 750 nm after acidification. Negative values were changed to zero
float nanometer
ABS_666a Background corrected absorbance measurement on spectrophotometer at wavelength 666 nm after acidification, 665 nm was used for ethanol extractions
float nanometer
Chl_mg/m2 Concentration of chlorophyll a
float milligramsPerSquareMeter
Pheo_mg/m2 Concentration of pheophytin a
float milligramsPerSquareMeter
Flags notes or problems encountered
string
Enumeration:
  • Negative Chloro: Negative Chloro
  • Negative phaeo: Negative phaeo
  • Weird: Weird

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